LAB HOME    NGHS HOME

 

CONTACT US
Search Help

Search for:

 
      Tests/Panels                

 

MICROBIOLOGY

To ensure the quality of Microbiology specimens, please follow the recommended collection procedures listed here:

  • NOTE: Please place all cultures containers in sealable plastic bags

  • Please label all specimens with name, date, and time collected


SPECIMEN TYPE:AFB (Acid Fast Bacilli)

CONTAINER: Sterile container with tight fitting top,

INSTRUCTIONS:

  • SPUTUM – Deep cough, morning.

  • URINE – Mid stream, early morning

  • WOUND – Sample of exudate or excised tissue.  Do NOT submit a swab. All specimens are processed in-house, with positive cultures being sent to State Laboratory for identification and sensitivity.


SPECIMEN TYPE: BLOOD

CONTAINER:

  • Two blood culture bottles for direct inoculation. If ARD is requested, special resin culture bottles must be used.

  • Cleanse top of bottle with single use alcohol swab only.

INSTRUCTIONS:

  • Cleanse skin over venipuncture site with 70% alcohol.  Follow with 2% Tincture of Iodine or Povidine Iodine in a circular motion – allow to remain on skin 1 minute.

  •  If palpation is needed, prep fingers in identical fashion.
  •  Perform venipuncture.

  • Place 5cc in each bottle for adults, 1 cc in each bottle for infants.

  • DO NOT Refrigerate. Do NOT leave bottles on counter until end of day or shift.

  • TRANSPORT IMMEDIATELY.


SPECIMEN TYPE: CSF

CONTAINER: Sterile, clean screw capped tube(s).  Follow physician’s instructions. 

INSTRUCTIONS:

  • To be collected by physician only.

  • TRANSPORT IMMEDIATELY.


SPECIMEN TYPE: GC (Neisseria Gonorrhoeae)

CONTAINER:
Sterile Swab
Jembec Plates (GC plates)

INSTRUCTIONS:

  • To be collected only by physician or nurse.

  • Use speculum without lubricant.

  • FEMALE: Swab high in vaginal canal

  • MALE: Insert swab well into urethra.  Streak Jembec plate at bedside. TRANSPORT IMMEDIATELY.


SPECIMEN: Herpes

CONTAINER: Collection kit obtained from Laboratory. 

INSTRUCTIONS:

  • Follow special instructions in kit.

  • Fill out form included in kit completely.

  • Sent to reference laboratory for processing.

  • TRANSPORT IMMEDIATELY.


SPECIMEN: THROAT (CULTURE)

CONTAINER: Culturette or Copan Swab

INSTRUCTIONS:

  • Depress tongue with depressor

  • Insert swab through mouth, being careful not to touch any other surface.

  • Roll swab over both tonsillar area and/or any visible throat crypts.  Especially swab any inflammed areas.

  • Return swab to holder.  Label with name, date and time.


SPECIMEN: SPUTUM

CONTAINER: Sterile container with tight fitting top

INSTRUCTIONS:

  • Obtain early morning specimen unless otherwise ordered. 

  • Have patient rinse mouth with water, clearing any nasal secretions before cough. 

  • Instruct patient to raise sputum by deep coughing. 

  • Determine adequate specimen: Should be more viscid (thick) than saliva, and usually will be colored or white but not clear.

  • 5ml is required.

  • TRANSPORT IMMEDIATELY.


SPECIMEN: STOOL FOR CLOSTRIDIUM DIFFICILE TOXIN

CONTAINER: Clean vial with NO preservatives

INSTRUCTIONS: Must be liquid stool; all formed stools will be rejected.


SPECIMEN: STOOL FOR CULTURE, O & P, WBC, ROTAVIRUS, GIARDIA/CRYPTOSPORIDIUM ANTIGEN

CONTAINER: Parapak - set containing Proto-fix & ETM (Can be used for culture, O&P, WBC, rotavirus and Giardia antigen.)

INSTRUCTIONS:

  • Collect specimen in bedpan (make sure specimen is free of urine) and transfer to media. 

  • Collect first morning specimen if possible. 

  • With infants, may collect from diaper or use rectal swab. Do NOT send diaper with absorbed specimen.

SPECIMEN - SURFACE WOUND:

  • COSPAN Transport Swab for aerobic culture.

  • COSPAN Transport Swab for anaerobic culture.

  • Open Lesion and express pus onto swab.

  • Transport immediately.

SPECIMEN - DEEP WOUND:

  • Sterile needle and syringe.

  • To be collected only by physician or at specific request of physician.

  • Cleanse skin.

  • If possible, aspirate encapsulated lesion with needle and syringe and transport to laboratory immediately with needle cover over needle.

URINE - MIDSTREAM:

  • Sterile container.

  • Catch urine in mid-stream. Instruct, assist patient.

  • Urine can be refrigerated up to 24 hours without change in count.

  • Transport immediately

URINE - CATHETERIZED:

  • Sterile container.

  • Decontaminate catheter line with alcohol swab. Aspirate urine through connector on sampling port with needle and syringe. Do not culture Foley catheter tips. Do not collect urine from Foley bag

  • Avoiding contamination, place in sterile container.

  • Transport immediately.

 

Print-Friendly Page

 

Contact Us     Welcome Letter    Meet Our Team     Billing Info      Forms      Specimen Collection/Handling
         Cytology          Genetic Testing
        Histopathology        Microbiology       Panels       Test Menu
Flow Cytometry      Therapeutic Drug Monitoring      Supplies         Home